4.2.2.3: Sample Adsorption
Interactions of the analyte with the capillary wall negatively affect zone broadening. In some cases, complete adsorption of the analyte to the capillary wall can occur. As most capillary tubing is made of borosilicate glass, this is most substantial with positively charged analytes that can interact strongly with the hydroxide groups on the surface of the glass. Prolonged sample-surface interactions can result in peak tailing and zone broadening.
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