Preliminaries

CHAPTER 1
Introduction, Chromatography Theory, and Instrument Calibration

CHAPTER 2
Basic Gas Chromatography

CHAPTER 3
Basic High Performance Liquid Chromatography

CHAPTER 4
Basic Capillary Electrophoresis

CHAPTER 5
Basic Mass Spectrometry

5.1 Introduction and History

5.2 Sample Introduction from GC and Analyte Ionization

-5.2.1 Analyte ionization

--5.2.1.1 Ionization Techniques for Solid Non-Volatile Analytes

--5.2.1.2 Ionization Techniques for Volatile Analytes Entering the MS from a GC
-- 5.2.1.2a Electron Ionization or Electron Impact (EI)

-- 5.2.1.2b Chemical Ionization (CI)

-5.2.1.3 Repulsion and Accelerator Plates, Slits, and Electronic Focusing Lens

5.3 The Introduction of Samples from HPLC

-5.3.1 Electro-Spray Ionization (ESI) Sample Introduction

-5.3.2 Matrix Assisted Laser Desorption/ Ionization, MALDI

-5.3.3 Proton Transfer Reaction Ionization (PTR)

-5.3.4 Fast Ion Bombardment (FAB)

5.4 The Introduction of Samples from a Capillary Electrophoresis System

5.5 Common Mass Filters (Mass Analyzers)

-5.5.1 Magnetic sector mass filter

-5.5.2 Quadrupole mass filter

-5.5.3 Quadrupole ion trap mass filter

-5.5.4 Time-of-Flight (TOF) mass filter

-5.5.5 Double Focusing Systems

-5.5.6 Fourier Transform Ion Cyclotron – Mass Spectrometry

-5.5.7 Orbitrap

-5.5.8 Tandem Mass Spectroscopy

5.6 Ion Detectors

5.7 Three-Dimensional Aspects of GC-MS

5.8 Summary

5.9 Questions

5.10 References

CHAPTER 6
Fragmentation and Interpretation of Spectra

CHAPTER 7
Proper Laboratory Protocol and Laboratory Experiments

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