Preliminaries

CHAPTER 1
Introduction, Chromatography Theory, and Instrument Calibration

CHAPTER 2
Basic Gas Chromatography

CHAPTER 3
Basic High Performance Liquid Chromatography

CHAPTER 4
Basic Capillary Electrophoresis

CHAPTER 5
Basic Mass Spectrometry

CHAPTER 6
Fragmentation and Interpretation of Spectra

CHAPTER 7
Proper Laboratory Protocol and Laboratory Experiments

7.1  Preliminary Information
7.1.1 Instrument and Instrumental Settings

7.1.1.a  Temperature Settings

7.1.1.b  Gas Flow

7.1.1.c.  Vacuum Chamber

7.1.2 Maintenance

7.1.3 Trouble Shooting

7.2  Preliminary Experiments:  Getting to Know Your Instrument
7.2.1  Autotuning the MS

7.2.2  Optimizing Analyte Separations with a Temperature Program

7.2.3  Obtaining a Linear Calibration Line

7.2.4  Electron (hard) versus Chemical (soft) Ionization.

7.3  Concept Illustrative Experiments
7.3.1 Advantages of GC-MS over GC

7.3.2  Advantages of GC over MS; cis- versus trans-

7.3.3 Advantages of GC over MS; Chiral separations

7.4  Analytical Experiments with an External Reference Standard Calibration
7.4.1  Caffeine Concentrations in Human Urine

7.4.2 Analysis of Cocaine Concentration on U.S. Currency

7.5  Analytical Experiments without an External Reference Standard; Conformational Identification without Quantification.

7.5.1  Identification of Components in Liquors and Distilled Spirits

7.5.2  Identification of Fragrances

7.5.3 SPME-GC-MS Analysis of Wine Headspace

7.5.4 An Extraction Procedure for the Investigation of Pesticide Residues on Strawberries

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