Paul H. Yancey, Whitman College

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III. RESEARCH at SEA (below)



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III. Deep-Sea RESEARCH AT SEA: updated 8/06 with Alvin information; see new VENTS page for 2007-08 Alvin photos

R/V Wecoma

R/V Thompson

R/V Atlantis

Pt. Lobos with Ventana ROV
ROV Nereus
DSRV Alvin (pre-2013 version)

Multicoring device

CTD device
Studying the deep sea requires properly outfitted research ships (R/V for research vessels), with cranes and winches. Examples of ones we have used are the Wecoma of Oregon State Univ., the Thomas G. Thompson of Univ. Washington, and the Atlantis of WHOI .
--Research with trawls and dredges is a round-the-clock process. It involves hours of free time as the net goes down then up. During this time we analyze samples and data, sleep, eat, or relax with books, movies, games, watching the sea. This is followed by intense teamwork when the net returns as the fishes and invertebrates have to be sorted, dissected, analyzed and preserved before they decay. See pictures below.

1. Deep-sea researchers may use manned submersibles such as:
--ALVIN (click for details at Woods Hole Oceanographic Institute) which we used in 2006 on an expedition to methane seeps off N. Calif. and Oregon with Dr. Lisa Levin (Scripps), and in 2007 and 2008 to hydrothermal vents on the Juan de Fuca Ridge with Dr. Ray Lee (WSU).
2. Researchers may also use remote-operated vehicles (ROVs) such as:
--the Ventana at the Monterey Bay Aquarium Research Inst. (MBARI), which provided us samples in 2003,
--the Oceanic Explorer (middle picture above), which we used on a 2001 expedition with Dr. Lisa Levin to methane seeps off N. California.
See pictures of Alvin and ROV operations below.
-The deepest-diving ROVs were:
--1) the Japanese Kaido, which ws used several times at the greatest ocean depth (Challenger Deep, Mariana Trench) in the 1990s. Unfortunately, it was lost at sea in Sept. 2003.
--2) The US hybrid Nereus, which also reached the deepest depths (Mariana Trench, 2009). Sadly, it too was lost in 2014 on our HADES expedition to the Kermadec Trench. SEE PHOTO HERE.

3. Relatives of the ROVs are the AUVs (autonomous underwater vehicles)--self-propelled robots carrying instruments that can rove the seas for long periods.

4. Other information:
--ALVIN was completely rebuilt 2010-2013.
--See LIVE camera images from MBARI's ROVs when they are in operation.
--Read more about deep-sea submersibles at PBS' NOVA site on the Abyss

D. CAMERAS and OTHER PERMANENT INSTRUMENTS: Other deepsea research is using cameras and other instruments on platforms on the seafloor, or on buoys at the surface. Examples: Mbari's Ocean Observing System (MOOS), the New Millenium Observatory (NeMO) on the Juan de Fuca ridge, and the Neptune Undersea network off western USA and Canada.

E. LANDERS, CORING DEVICES, WATER SAMPLERS, Etc.: There are also a variety of other devices which can be deployed into the deep with a crane and winch on research ships, or dropped free-fall to the seafloor (to be retrieved by sending a sonic signal to tell the device to release its weights). One example is a multicoring device (picture above) which plunges into the seafloor and collects cores of sediment. Another is a Conductivity-Temperature-Depth (CTD) sensor and water sampler (see picture, above). Both of these remain tethered to the winch cable. There are also specialized free-falling LANDERS and ELEVATORS: see HADAL PAGE for examples

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trawltrawl sunrise sunset dolphin catchcatch2
1. Operating otter trawl (1996/2009)
After deploying the "otter" trawl, we took 2 hours to feed 6000m (almost 4 miles) of cable out to insure the net hit bottom at 2900m. Then we trawled on the bottom for 2 hrs, then took another 2 hrs to haul it in
2. Sunrise at sea (4/97)
--we work around the clock in "watches" (like shifts on land) when trawling or using an ROV.
3. Sunset at sea (4/2014, South Pacific)
--I thought I saw the famous "green flash" after one sunset. However, astronomer Andrew Young of SDSU reports this flash, though often real, may sometimes be an illusion resulting from red sunset light bleaching out our red retinal cones, leaving our eyes to see yellow light as green (New Scientist, 20-June-98, p.5)
4. Porpoises "surfing"
Dolphins and porpoises occasionally ride the ship's bow wave. In 1992 it was shown that this saves these animals considerable locomotory energy (of course, they may also be having fun!). Click SURFACE button at bottom of page to get more pictures and videos of porpoises in the wild
5. Typical catch
of abyssal invertebrates (sea cucumbers, seaspiders, shrimp, anemones, soft urchin, etc.), and rattail/grenadier fish from a deepsea trawl
n1 .
6. ROV on deck of Thompson
Oceanic Explorer
sits on the deck of the ship, 2001
7. ROV launch
--Nereus picked up by crane and lifted to the water, 2014
8. ROV manipulator at work
--arm collects specimens and experimental equipment
9. ALVIN launch
--the A-frame drops it slowly into the water, while swimmers (specially trained divers) wait in an Avon boat to remove the ropes
10. Inside ALVIN
-- 1 pilot and 2 "observers" fit snugly inside the titanium sphere, each with a porthole first dive!* [photo of me by Tina Treude]
11. ALVIN at work
-- the pilot deftly uses the leftside manipulator arm to scoop up sediment and seep clams. During one dive, a cheeky squid attacks the arm!
12. ALVIN returns
--swimmers reach the Alvin after it surfaces to get the sub ready for recovery
13. Alvin Recovery 1
-- the swimmers perform the sometimes-dangerous task of hooking the A-frame ropes to the Alvin
14. Alvin Recovery 2
--after securing ALVIN, the swimmers jump off the sub to swim back to the Avon
15. Alvin "baptism"
--after returning from your first dive in ALVIN, you are ceremoniously greeted with a bucket of icewater and a seawater hosedown [photo by Andrew Thurber]

*MY FIRST ALVIN DIVE: Although I have been doing deep-sea research for almost 25 years, until 2006 I had not been down in a research submersible because my deep-sea research started on fish, which are much easier to catch by net than by submersible. However, my recent work has been on invertebrates, and submersibles and ROVs are great for collecting many of those. Thanks to Chief Scientist Lisa Levin (Scripps), in July 2006 I spent 8 hours in the Alvin, diving to 906m (about 3000ft) at Hydrate Ridge's East Basin off Oregon. [See my Seeps and Vents Page for more information and pictures.] Tina Treude (USC) and I dove with pilot Gavin Eppard. On the morning of our dive, it was touch-and-go on the weather, as the winds were near 25 knots, the unsafe limit. Finally we got the go-ahead. After we were sealed in and released into the water, we bounced around in the high waves for a bit, then Gavin flooded the ballast tanks and we began to sink slowly. It took about 45 min to reach 880 m. During that time, in which the sub lights are off, pure magic appeared out our windows as the sunlight faded into night. The ocean became a galaxy of flashing lights, far more than you ever see in videos! I could see luminescent jellies, squid, 4ft-long siphonophores, shrimp, and even fish blinking their headlights as they darted by. [See my Midwater Page for examples of these animals]
Finally we hit bottom and began our real work of collecting tubecores of gas-oxidizing bacteria and methane hydrate, rocks, and animals. Gavin was incredibly adept with the sub manipulators, moving the tubecores in and out of the sub holders and seafloor sediment with ease. We saw mounds of frozen hydrate; grey, white and orange bacterial mats; clambeds; carbonate rocks; sponges; searobins, eelpouts and hagfish; large Neptunea snails tracking across the mud; and mushroom corals. As we worked, larvaceans, ctenophores, jellies and fish drifted or swam past us. We also saw mysterious rings that might be methane hydrate (frozen natural gas) in a form that we've never heard described before.
About half-way through, the real prize appeared--a mysterious transparent squid with tentacles sprouting up from its head rather than in front as in 'normal' squid. I tentatively identified it as a Teuthowenia-type squid. The creature stayed by us for several minutes, allowing us a great look. As it got close to us, its head and tentacles turned red, then it darted off across my window.
At one hydrate site, Gavin used the sub manipulator to grab a rock. Upon our return, we found it to be a beautiful vent rock, lined with crystalline chimneys through which gas once bubbled. Later, he also grabbed a rock composed mostly of shells.
Upon finishing our collecting at the designated target areas, we had an hour of battery power left, so Gavin took us exploring down slope to see if there were undiscovered hydrate sites. He let each of us take a 5-min turn at the helm (actually, a joystick with 3 dimensions of control). While I was driving, we did come across a large bacterial mat around carbonate rocks. We eventually reached 906m before turning back.
Finally it was time to blow the ballast, drop the lead weights, and head up. Gavin loaded Led Zepplin into the sub stereo, turned off the lights, and again we rose through a luminescent fairyland. Finally we bounced to the surface and found ourselves in high waves, seesawing madly. It took some time to bring us safely aboard, and we found later that the winds were above the safe level. Once secured on board, we climbed out, and I was treated to the traditional bucket of icewater on my head and a hosedown with seawater!
All told, the dive was one of the highlights of my life. I still marvel at the skills of the pilot, swimmers, and all the other Alvin teammembers...and the entire crew of the Atlantis. Working with the other scientists was also a pleasure. My thanks to everyone!

ADDENDUM 2008: Thanks to collaborator Ray Lee of Washington State University, I dove again in Alvin in July 2008, this time to 2200m at the Faulty Towers hydrothermal vent field on the Juan de Fuca Ridge. This dive was truly otherworldly. Vent chimneys (known as white smokers) up to 70 ft (22m) rose above the seafloor spewing warm mineral-laden water. At the base of these towers, we came within sampling range of black smokers with water up to 600F (over 300C).

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Movie of methane vent
, near which hydrogen sulfide is produced by microbes
PRESSURE: High hydrostatic pressure (from the weight of the water column overhead) squeezes anything with an air chamber, such as fishes with swimbladders. Many deep-sea fishes do have fat-filled or vestigial swimbladders to avoid this problem, but some do have air-filled ones. These require considerable energy to inflate, and they expand (often lethally) when the fish is hauled to the surface. Pressure does not cause large-scale compression of most deepsea life due to the absence of air chambers, but it affects all life at a microsopic scale. It forces water molecules to stay densely bound to charged molecules, which interferes with critical binding events in cells (such as an energy-yielding molecule binding to an enzyme). For pressure adaptations, see below.

The HIGHEST PRESSURES are found in the Challenger Deep of the Mariana Trench in the western Pacific. This trench is almost 36,000 ft or 11,000 m deep with almost 1100 atm of pressure. The Mariana Trench has been explored by only 4 submersibles : 1) briefly by the manned sub Trieste in 1960; 2) in the 1990s by a Japanese unmanned ROV, "Kaiko", which was unfortunately lost at sea in Sept. 2003; 3) by the US unmanned hybrid ROV Nereus in 2009; and 4) by James Cameron in his DEEPSEA CHALLENGER one-man sub in 2012. For depth limits of submersibles and life, see the Smithsonian's How Deep Can They Go page, and Extreme Science's Deep Ocean Creatures page.
In 2014, we explored the Kermadec Trench with the Nereus, down to 10,000m.

SULFIDE: Deep-sea animals at hydrothermal vents and gas seeps face another stress: high levels of hydrogen sulfide (H2S), a gas normally toxic to animals. However, giant tubeworms and certain clams and mussels at vents and seeps rely on sulfide-metabolizing bacterial symbionts in their internal tissues. Somehow the animals avoid sulfide toxicity--by binding sulfide to special proteins or converting it into non-toxic molecules. For our work on sulfide adaptations, see section 2 below. For pictures, see Seeps and Vents page

map cupbefore bladder
1. Trawl and Alvin dive sites off Oregon; click here for schematic diagram. [3-D images of the seafloor can be see in Scientific American June 1997 (Oregon coast on p.84)] 2. Styrofoam head before and after a trip on the ALVIN
Hydrostatic pressure increases by 1 atm with each 10m of depth. This shows effects of 50 to 90 atm pressure on a styrofoam head taken to 520m and 906m on the Alvin (images at same scale)
3. Swimbladder decompression
--a rattail hauled for 2 hrs to surface from 2000m. The air-filled bladder cannot be adjusted fast enough for low pressure, and it expands out the mouth. Eyes are also pushed out by expanding air.
4. A transparent squid visits Alvin.
Squids and most animals in the oceans do not have air chambers in them, so they do not suffer from large-scale pressure effects. However, they do have to cope with microscopic effects on proteins and membranes; see OUR PRESSURE RESEARCH below.

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High pressure traps water molecules at a high density around charged molecules, interfering with critical binding events in cells involving proteins. Dr. Joe Siebenaller of L.S.U. and other researchers have found that many proteins in deep-sea fishes somehow compensate for this effect. However, not all deep-sea proteins are pressure resistant. Pressure also makes membranes more rigid, impairing transport functions, etc. Researchers have found that deep-sea organisms have unsaturated lipids in their membranes to "loosen" them up. Unsaturated lipids (an example is vegetable oil) have mixed C-C single bonds and C=C double-bonds that prevent the lipid chains from stacking tightly together, making the lipid or fat more liquidy. In contrast, saturated lipids have all C-C single bonds that stack together, making the lipid or fat more rigid (an example is butter).

In my laboratory, we have found that deep-sea fishes and some invertebrates have the highest known levels of trimethylamine oxide or TMAO (see below). This is a common compound in many marine animals, used to help maintain water balance against the high salinity of the sea. If your computer had "RealOdor" or "QuickSmell" technology, you'd recognize TMAO--it and its breakdown product, TMA, are what makes marine animals smell fishy. TMAO is also a stabilizer of proteins (helping these biological molecules remain functional when perturbed), and we have extensive evidence (see example below) that it may be very high in these animals in order to help pressure-sensitive proteins overcome pressure inhibition (perhaps by helping to remove dense water from charged molecules). See references by Gillett, Kelly, Samerotte and Yancey below, New Scientist news story (1999), and BBC story (2014) about a possible depth limit for fishes based on TMAO.

--We analyzed deep clams from cold seeps and found they have an unusual compound first reported by Alberic and Boulegue in 1990: serine-phosphoethanolamine-X (where X is an unknown). We discovered that this compound increases with depth in clams from 1100 to 6400m depth. It may help protect proteins from pressure effects (Fiess et al.).

RIGHT: Structure of TMAO; and GRAPH of Effects of TMAO on Lactate Dehydrogenase (LDH) from a rattail (=grenadier) fish. After 8 hr at 1000 atm, this enzyme in water lost much of its activity, but with TMAO it lost much less. LDH is an enzyme that is crucial for burst activity in muscles. TMAO.

RIGHT: Examples of laboratory high-pressure devices that we've used.

Click here for Joe Siebenaller's (LSU) chamber

George Somero's (Stanford) chamber for measuring enzyme activity under pressure
Jeff Drazen's (U. Hawai'i) trap for large fish.
Ray Lee's (WSU) chambers for small animals


--We are also investigating the roles of unusual sulfur osmolytes in animals from hydrothermal vents and gas seeps. Researchers in France (Alberic, Pranal, Pruski, Fiala-Medioni and others) found these animals to have large amounts of hypotaurine and thiotaurine, rare compounds related to the very common marine osmolyte taurine. [Taurine is also crucial for mammalian brain development and is a major ingredient in many sports/energy drinks.] These all contain sulfur, and may help in transporting sulfides to the bacterial symbiotes that these animals rely on (see Vents/Seeps page), or may protect the animals from toxic sulfide. Recently we found that snails and limpets, as well as the heat-loving paralvinellid worms, from the Juan de Fuca Ridge vents contain hypotaurine and thiotaurine at high levels even though they don't have internal symbionts. We have also found that animals (with or without symbionts) exposed to the highest sulfide levels have highest levels of hypotaurine and thiotaurine (Rosenberg et al. 2006; Brand et al. 2007; Yancey et al. 2009).

We discovered the sulfur compound methyltaurine as a dominant osmolyte in Lamellibrachia seep tubeworms. Its function is unknown, but as a methylamine, it may help counteract pressure effects (Yin et al. 2000).


  • Gillett, M.B., J.R. Suko, F.O. Santoso, P.H. Yancey (1997). Elevated levels of trimethylamine oxide in muscles of deep-sea gadiform teleosts: a high-pressure adaptation? J. Exper. Zool. 279:386-391
  • Kelly, R.H., P.H. Yancey (1999). High contents of trimethylamine oxide correlating with depth in deep-sea teleost fishes, skates, and decapod crustaceans. The Biological Bulletin 196:18-25 ; PDF version here
  • Yancey, P.H., J.F. Siebenaller (1999). Trimethylamine oxide stabilizes teleost and mammalian lactate dehydrogenases against inactivation by hydrostatic pressure andtrypsinolysis. J. Exper. Biol. 202:3597-3603; News Story: Dec. 11 '99 New Scientist (p.22)
  • Yin, M., H.R. Palmer, A.L. Fyfe-Johnson, J.J. Bedford, R.A. Smith, P.H. Yancey (2000). Hypotaurine, N-methyltaurine, taurine, and glycine betaine as dominant osmolytes of vestimentiferan tubeworms from hydrothermal vents and cold seeps. Phys. Biochem. Zool. 73: 629-637
  • Yancey, P.H., A.L. Fyfe-Johnson, R.H. Kelly, V.P. Walker, M.T. Auñón (2001). Trimethylamine oxide counteracts effects of hydrostatic pressure on proteins of deep-sea teleosts. J. Exp. Zool. 289:172.
  • Yancey, P.H., W. R. Blake, J. Conley (2002). Unusual organic osmolytes in deep-sea animals: adaptations to hydrostatic pressure and other perturbants. Comp. Biochem. Physiol. A, 133 (3): 667-676 (click on vol. 133)
  • Yancey, P.H., W. R. Blake, J. Conley, R.H. Kelly (2002). Nitrogenous solutes as protein-stabilizing osmolytes: counteracting the destabilizing effects of hydrostatic pressure in deep-sea fish. In: Nitrogen Excretion in Fish (Proc. Internatl. Congr. Biol. Fish), Wright, P.A. and D. MacKinlay (eds.).
  • Fiess, J., H.A. Hudson, J.R. Hom, C. Kato, P.H. Yancey (2002). Phosphodiester amine, taurine and derivatives, and other osmolytes in vesicomyid bivalves from cold seeps: correlations with depth and symbiont metabolism. Cahiers de Biologie Marine 43: 337-340
  • Yancey, P.H., M.D. Rhea, D. Bailey, K. Kemp (2004). Trimethylamine oxide, betaine and other osmolytes in deep-sea animals: depth trends and effects on enzymes under hydrostatic pressure. Cell Molec. Biol. 50: 371-376
  • Rosenberg, N.K., R.W. Lee, P.H. Yancey (2006). High contents of hypotaurine and thiotaurine in hydrothermal-vent gastropods without thiotrophic endosymbionts. J. Exp. Zool. 305A: 655-662.
  • Brand*, G.L., R.V. Horak*, N. LeBris, S.K. Goffredi, S.L. Carney, B. Govenar, P.H. Yancey (2007). Hypotaurine and thiotaurine as indicators of sulfide exposure in bivalves and vestimentiferans from hydrothermal vents and cold seeps. Mar. Ecol. 28: 208-218.
  • Samerotte*, A.L., J.C. Drazen, G.L. Brand*, B.A. Seibel, P.H. Yancey (2007). Contents of trimethylamine oxide correlate with depth within as well as among species of teleost fish: an analysis of causation. Phys. Zool. Biochem. 80: 197-208
  • Yancey, P.H., J. Ishikawa*, B. Meyer*, P. Girguis, R. Lee (2009). Hypotaurine and thiotaurine in polychaetes without endosymbionts from hydrothermal vents: correlation with sulfide exposure. J. Exp. Zool. 311A:439-447.
  • Laxson*, C., N. E. Condon, J. C. Drazen, and P.H. Yancey (2011). Decreasing urea:methylamine ratios with depth in Chondrichthyes: A physiological depth limit? Physio. Biochem. Zool.84:494-505 online pre-publication
  • Jamieson, A., P.H.Yancey (2012). On the validity of the Trieste flatfish: dispelling the myth. Biol. Bull 222:171-175
  • Yancey, P.H., M. Gerringer*, A.A. Rowden, J.C. Drazen, A. Jamieson (2014). Marine fish may be biochemically constrained from inhabiting the deepest ocean depths. Proc. Natl. Acad. Sci. USA Mar. 3; see news story here
  • Ichino, M C., M.R Clark, J.C. Drazen, A. Jamieson, D.O.B. Jones, A.A. Rowden, T.M. Shank, P.H. Yancey and H.A. Ruhl (2015). The distribution of benthic biomass in hadal trenches: a modelling approach to investigate the effect of vertical and lateral organic matter transport to the seafloor. Deep-Sea Res. I. 100:21-33.
  • Yancey, P.H, and J.F. Siebenaller (2015). Co-evolution of proteins and solutions: protein adaptation versus cytoprotective micromolecules and their roles in marine organisms. J. Exp. Biol. 218:1880-1896.
  • Yancey, P.H (2015). Organic osmolytes in elasmobranchs. IN: Fish Physiology (R.E. Shadwick, A.P. Farrell, C.J. Brauner, eds), Vol. 34B; Academic Press.
  • Divino, J.N., M. Monette, S.D. McCormick, P.H. Yancey, K.G. Flannery*, M.A. Bell, F.A. von Hippel, and E.T. Schultz (2016). Osmoregulatory physiology and rapid evolution of salinity tolerance in a recently introduced lake population of Threespine Stickleback. Evolutionary Ecology Research. 17:179-201.
  • Linley, T., M. Gerringer, P.H. Yancey, J.C. Drazen, C. Weinstock*, A. Jamieson (2016). Fishes of the hadal zone including new species, in situ observations and depth records of Liparidae. Deep-Sea Research I, 114: 99-110.
  • Mills S., Leduc D., Drazen J. C., Yancey P., Jamieson A. J., Clark M. R., Rowden A. A., Mayor D. J., Piertney S., Heyl T., Bartlett D., Bourque J., Cho W., Demopoulos A., Fryer P., Gerringer M., Grammatopoulou E., Herrera S., Ichino M., Lecroq B., Linley T. D., Meyer K., Nunnally C., Ruhl H., Wallace* G., Young C. and Shank T. M. (2016). 10,000 m under the sea: an overview of the HADES expedition to Kermadec Trench. IN: B. Golder and A. Connell (Eds.) Proceedings of Kermadec–Discoveries and Connections (pp 36–38). The Pew Charitable Trusts
  • Gerringer, M.E., Drazen, J.C., Yancey, P.H. (2017). Metabolic enzyme activities of abyssal and hadal fishes: pressure effects and a re-evaluation of depth-related changes. Deep-Sea Res. I 125: 135–146

3. Protein Contents and Anatomy of Midwater (Mesopelagic) Fishes (1980s)

We discovered that the muscles of midwater fishes (such as viperfish) have extremely low protein contents: 5-10% protein, compared to 15-20% in a surface (epipelagic fish, such as a tuna). We also discovered that some of these deep fish have an unusual buoyant gelatinous layer around their spines or under their skins:

  • Siebenaller, J.F., P.H. Yancey (1984). The protein composition of white skeletal muscle from mesopelagic fishes having different water and protein contents. Mar. Biol. 78: 129-137
  • Yancey, P.H., R. Lawrence-Berrey, M. D. Douglas (1989). Adaptations in mesopelagic fishes. I. Buoyant glycosaminoglycan layers in species without diel vertical migrations. Mar. Biol. 103: 453-459
  • Yancey, P.H., T. Kulongoski, M.D. Usibelli, R. Lawrence-Berrey, A. Pedersen (1992). Adaptations in mesopelagic fishes. II. Protein contents of various muscles and actomyosin contents and structure of swimming muscle. Comp. Biochem. Physiol. 103B: 691-697
See also MIDWATER PAGE for pictures of the gelatinous layer and fishes
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