PAUL H. YANCEY

Professor of Biology
Carl E. Peterson Endowed Chair of Sciences

Biology Dept., Whitman College, Walla Walla WA 99362 USA
Curriculum Vitae

Click here for
TEACHING :
ANIMAL PHYSIOLOGY, MARINE BIOLOGY, BIOETHICS,
[HUMAN ANATOMY & PHYSIOLOGY
]

Click here for
RESEARCH:
ENVIRONMENTAL STRESSES and ORGANIC OSMOLYTES
Click for a full list of Prof. Yancey's experiences and publications

BIOGRAPHY

Prof. Yancey's hobbies include photography, woodworking, stained glass, hiking, gardening.

Current Research:
OSMOLYTES in:
Corals under Stress; European Eels and Salmon; Sports Drinks;
Deep Sea and Pressure Adaptations
--HADES - NSF grant

. moorea
In the submersible Alvin (2006); with a deep-sea cuskeel (2009); on the SEA
Semester sailing ship at Moorea, French Polynesia (2013)


TEACHING: Prof. Yancey's courses and other teaching activities are listed below, and can be accessed by clicking the blue links:

 

Courses taught:

 

Educational sites on adaptations:

 

Physiology*
310

Human Anatomy & Physiology 120

Marine Biology
178, 179: Non-majors
278, 279: Major elective

Bioethics
401/402

Student Research
489,490,498

 

DEEP-SEA
Educational Website

African & Australian Animal
Adaptations Website

*Dr. Yancey is a co-author of a 2005 (1st ed.) and 2013 (2nd ed.) TEXTBOOK: ANIMAL PHYSIOLOGY, by Sherwood, Klandorf, Yancey (Cengage)

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RESEARCH: Prof. Yancey's research is described below, with additional information found by clicking the blue topics:

 

OVERVIEW
Most of the research in Prof. Yancey's laboratory focuses on Organic Osmolytes. Osmolytes are compounds that cells may accumulate when they are under dehydrating osmotic stress. Such stresses include high salinity (as in seawater, or in the interior of the mammalian kidney), high evaporation (as in deserts), freezing, dietary imbalances, and diseases (e.g., osmotic stresses caused by diabetes mellitus). Organic osmolytes are certain compounds which can be built up in cells to elevate osmotic pressure and which, unlike salt ions, do not disrupt cellular macromolecules. In addition to regulating cellular water balance, many osmolytes have cytoprotective properties such as stabilizing cellular proteins against denaturing chemicals like urea, temperature, and pressure.

More information can also be found by at Prof. Yancey's SENIOR RESEARCH page.
IN THE NEWS:
hades
A biochemical depth limit for fishes?PNAS paper 3/2014
2012 HADES news Science; Science News
2012 Cover Story: Trieste flatfish, Biol. Bulletin
2012 Giant trench amphipod story
2010 hagfish slime story in J. Exp. Biol.
1999 New Scientist on TMAO & pressure


TEAM OSMOLYTE

.amphi giant.

I. REVIEW and COMMENTARY PAPERS

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RECENT and CURRENT OSMOLYTE RESEARCH:

1) DEEP-SEA animals and PRESSURE: Osmolyte roles in animals in depth/pressure adaptation
--NSF international HADES Program: 2011-2015 --with Tim Shank, WHOI, Jeff Drazen, U. Hawai'i (co-PIs); international collaborators: Alan Jamieson, U. Aberdeen; Ashley Rowden, NIWA NZ; and several others including Doug Bartlett of Scripps Inst. Oceanogr. and James Cameron's DEEPSEA CHALLENGE
--See NEWS stories in Science and Science News.

2) CORAL Osmolytes and Stress Factors
--A) CRYOPRESERVATION of coral larvae and adults for re-seeding decimated reef areas --with Mary Hagedorn, Smithsonian Institution
--B) SALINITY/pH adaptations of corals in ojos de agua near Yucatan's Mesoamerican Reef [pics here] --collaboration with Adina Paytan, Elizabeth Derse (UCSC) and Mario Rebolledo-Vieyra, Laura Hernandez (Centro de Investigacion Cientifica de Yucatan)

3) BRAIN CELLS and BETAINE [osmolyte]: Protective properties in exercise-related stress?
--Leena Knight (Whitman); Stuart Craig, Kirsti Tiihonen (Danisco/DuPont)


4) Anguilla EELS and SALMON
Osmolytes--with Gordon Cramb, University of St. Andrews UK

5) Hydrothermal-Vent & Cold-Seep animals
--Roles of Hypotaurine/Thiotaurine [Osmolyte derivatives];
--with Ray Lee, Washington State U.; Peter Girguis, Harvard U.)


6) Hagfish Slime
: Osmolytes role in swelling?--with Doug Fudge, Julia Herr (U. Guelph); news story

 

....
LEFT: Trimethylamine oxide (TMAO)
RIGHT: Hypotaurine
[add O or an SH to the S-group to
form taurine or thiotaurine, respectively]

DETAILS:
Prof. Yancey and others have found that some osmolytes, especially methylamine types such as TMAO (left), can actually stabilize proteins and counteract destabilizing effects of perturbants such as urea, salt, temperature and pressure. TMAO has a breakdown product, TMA (trimethylamine), that makes marine animals smell "fishy." Methylamines are high and appear to protect proteins in
i) sharks and relatives, which also have the perturbing compound urea as an osmolyte;
ii) mammalian (including human) kidneys, which must concentrate urea as a perturbing waste;
iii) deep-sea animals which must cope with protein disturbances from high pressure. Our discovery of TMAO's role in the deep sea was featured in a New Scientist news story in 1999 and in a 2012 cover story of Science News. See Deep-sea Fish page for pictures and Deep-Sea Research page for research details.

--Stabilizing properties of osmolytes may have practical applications, e.g., Welch and colleagues have shown that TMAO and other osmolytes can prevent the damaging protein of "mad-cow" disease from forming, and can cause the malformed protein of cystic fibrosis to fold properly. (Dr. Yancey assisted in one of the latter studies; see Howard et al. reference below in Research Area 2.)

--We are also studying the role of osmolyte-type solutes in animals at hydrothermal vents and gas seeps, which have high levels of hydrogen sulfide, a gas toxic to most animals. A major osmolyte in shallow-water marine invertebrates such as clams and crabs is taurine. Taurine is also essential for mammalian brain development, and is the primary ingredient in many so-called energy or sports drinks (hint: the name taurine is derived from Taurus [bull]). Researchers in France have found high levels of the taurine derivatives hypotaurine and thiotaurine in clams, mussels and tubeworms which have sulfide-oxidizing bacterial symbionts. Thiotaurine, a product of hypotaurine and sulfide, may be a mechanism to prevent sulfide toxicity. We have found hypotaurine and thiotaurine in vent snails, limpets and heat-loving paralvinellid worms without symbionts, and shown that thiotaurine levels vary with sulfide exposure in these animals kept in laboratory pressure chambers. See Seeps and Vents page for pictures and Deep-Sea Research Page for research details.

--Other researchers have found that the common osmolyte of marine algae, DMSP(dimethylsulfonoproprionate), breaks down into the gas DMS (dimethylsulfide), which is largely responsible for the "smell of the sea" that evokes emotional responses to the ocean. DMS is also thought to trigger the seeding of clouds, in what may be a global temperature negative feedback process. This is one of the postulates of the so-called Gaia hypothesis, which suggests that global warming will cause more DMS production, which via cloud formation may cool the planet.
-- We have recently been working on DMSP and other osmolytes in coral animals and their symbionts, with Dr. Mary Hagedorn, who is hoping to cryopreserve coral larvae for potential re-seeding of decimated reef habitats.

 

PUBLICATIONS: Click for a full list of Prof. Yancey's publications. Papers are listed below by theme/area

I. REVIEW and COMMENTARY ARTICLES

A. REVIEW ARTICLES on osmotic balance and cytoprotection using osmolytes (RED = recommended reading for overview on osmolytes) [Primary research articles are below]:

  • Yancey, P.H., M.E. Clark, S.C. Hand, R.D. Bowlus, G.N. Somero (1982). Living with water stress: evolution of osmolyte systems. Science 217: 1214-1222 (An I.S.I. Citation Classic--see Yancey 1993 under B. COMMENTARY Articles below)
  • Somero, G.N., P.H. Yancey (1978). Evolutionary adaptations of Km and kcat values: fitting the enzyme to its environment through modifications in the amino acid sequences and changes in the solute environment of the cytosol. Symp. Biol. Hungar. 21: 249-276
  • Yancey, P.H. (1985). Organic osmotic effectors in cartilaginous fishes. IN: Transport Processes, Iono- and Osmoregulation (R. Gilles, M. Gilles-Ballien, eds), Springer-Verlag
  • Yancey, P.H. (1994). Compatible and counteracting solutes. In: Cellular and Molecular Physiology of Cell Volume Regulation, Strange, K. (ed.), CRC Press, Boca Raton.
  • Somero, G.N., P.H. Yancey (1997). Osmolytes and cell volume regulation: physiological and evolutionary principles. In: Handbook of Physiology, Sec. 14; Hoffman, J. F. and J.D. Jamieson (eds)., Oxford University Press.
  • Yancey, P.H. (2001). Water stress, osmolytes and proteins. Amer. Zool. 41: 699-709.
  • Yancey, P.H. (2001). Nitrogenous solutes as osmolytes. Fish Physiology Vol. 20: Nitrogen Excretion (P.Wright, P. Anderson, eds). Academic Press, pp 309-341.
  • Yancey, P.H., W. R. Blake*, J. Conley*, R.H. Kelly* (2002). Nitrogenous solutes as protein-stabilizing osmolytes: counteracting the destabilizing effects of hydrostatic pressure in deep-sea fish. In: Nitrogen Excretion in Fish (Proc. Internatl. Congr. Biol. Fish), Wright, P.A. and D. MacKinlay (eds.).
  • Yancey, P.H. (2003). Proteins and counteracting osmolytes. Biologist 50: 126-131
  • Yancey, P.H. (2004). Compatible and counteracting solutes: protecting cells from the Dead Sea to the deep sea. Science Progress 87: 1-24
  • Yancey, P.H. (2005). Organic osmolytes as compatible, metabolic, and counteracting cytoprotectants in high osmolarity and other stresses. J.Exp. Biol. 208: 2819-2830
B. COMMENTARY ARTICLES
  • Weiler, C.S., P.H. Yancey (1989). Dual-career couples and science: opportunities, challenges and strategies. Oceanography 2: 28-31; PDF here
  • Weiler, C.S., P.H. Yancey (1992). Dual-career couples and academic science. J. Coll. Sci. Teach. 21: 217-222; PDF here
  • Yancey, P.H. (1993). Micromolecules that help macromolecules in dehydration [commentary written for I.S.I. Citation Classic recognition]. Curr. Contents Life Sci; PDF here
  • Sandquist, J., L. Romberg, P. Yancey (2013 in press). Life as a professor at a small liberal arts college. Mol. Biol. Cell

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II. MARINE / COMPARATIVE PHYSIOLOGY: ADAPTATIONS to SALINITY, TEMPERATURE, SULFIDE, PRESSURE:

A. SHALLOW OCEANS: SHARKS and relatives, BONY FISH, and CORALS; plus FROGS (*undergraduate co-authors):
(For DEEP-SEA animals, see next section below)

  • Yancey, P.H., G.N. Somero (1978). Urea-requiring lactate dehydrogenases of marine elasmobranch fishes. J. Comp. Physiol. 125: 135-141
  • Yancey, P.H., G.N. Somero (1979). Counteraction of urea destabilization of protein structure by methylamine osmoregulatory compounds of elasmobranch fishes. Biochem. J. 182: 317-323
  • Yancey, P.H., G.N. Somero (1980). Methylamine osmoregulatory compounds in elasmobranch fishes reverse urea inhibition of enzymes. J. Exp. Zool. 212: 205-213
  • Altringham, J.D., P.H. Yancey, I.A. Johnston (1982). The effects of osmoregulatory solutes on tension generation by dogfish skinned muscle fibres. J. Exp. Zool. 96: 443-445
  • Bedford, J.J., J.L. Harper, J.P. Leader, P.H. Yancey, R.A.J. Smith (1998). Tissue composition of the elephant fish, Callorhynchus milli: Betaine is the principal counteracting osmolyte. Comp. Biochem. Physiol. 119B: 521-526 (see picture at right)
  • Yancey, P.H., J. Ruble*, J.D. Valentich (1991). Effect of chloride secretagogues on cyclic AMP formation in cultured shark (Squalus acanthias) rectal gland epithelial cells. Bull. Mt. Des. I. Biol. Lab.13: 51-52
  • Fuery, C.J., P.V. Attwood, P.C. Withers, P.H. Yancey, J. Baldwin, M. Guppy (1997). Effects of urea on M4-lactate dehydrogenase from elasmobranchs and urea-accumulating Australian desert frogs. Comp. Biochem. Physiol. 117B: 143-150
  • Steele, S.L., P.H. Yancey, P.A. Wright (2004). Dogmas and controversies in the handling of nitrogenous wastes: Osmoregulation during early embryonic development in the marine little skate Raja erinacea; response to changes in external salinity. J. Exp. Biol. 207: 2021-2031
  • Steele, S.L., P.H. Yancey, P.A. Wright (2005). Evidence for an extra-hepatic ornithine-urea cycle and osmoregulatory strategies in response to low salinity in the little skate, Raja erinacea. Physiol. Biochem. Zool. 78: 216-226
  • Fiess, J.C., A. Kunkel-Patterson*, L. Mathias*, L.G. Riley, P.H. Yancey, T. Hirano, E.G. Grau (2007). Effect of environmental salinity and temperature on osmoregulatory ability, organic osmolytes, and plasma hormone profiles in the Mozambique tilapia (Oreochromis mossambicus). Comp. Physiol. Biochem. 146A: 252-264
  • Hagedorn, M., V.L. Carter, S. Ly*, R.A. Andrell*, P.H. Yancey, J.A. Leong, F.W. Kleinhans (2010). Analysis of internal osmolality in developing coral larvae, Fungia scutaria. Phys. Biochem. Zool. 83: 157-166
  • Yancey, P.H., M. Heppenstall*, S. Ly*, R.M. Andrell*, R.D. Gates, V.L. Carter, M. Hagedorn (2010). Betaines and dimethylsulfoniopropionate (DMSP) as major osmolytes in Cnidaria with endosymbiotic dinoflagellates. Phys. Biochem. Zool. 83: 167-173
  • Kalujnaia, S., S. Gellatly, N. Hazon, A. VillaseƱor*, P.H. Yancey and G. Cramb (2013). Tissue distribution of inositol monophosphatase (IMPA) isoforms in two euryhaline teleosts, the European eel (Anguilla anguilla) and the Nile tilapia (Orechromis niloticus); the effects of SW-acclimation on isoform expression and inositol production. Amer. J. Physiol. In press; Epub June 5


Elephant fish, New Zealand

Skate egg case
fungia
Fungia coral, whose larvae we are
trying to cryopreserve in
Mary Hagedorn's lab at U. Hawaii

 

B. DEEP-SEA ANIMALS -- PRESSURE; HYDROTHERMAL VENTS and COLD SEEPS (*undergraduate co-authors):

  • Siebenaller, J.F., P.H. Yancey (1984). The protein composition of white skeletal muscle from mesopelagic fishes having different water and protein contents. Mar. Biol. 78: 129-137
  • Yancey, P.H., R. Lawrence-Berrey*, M. D. Douglas* (1989). Adaptations in mesopelagic fishes. I. Buoyant glycosaminoglycan layers in species without diel vertical migrations. Mar. Biol. 103: 453-459
  • Yancey, P.H., T. Kulongoski*, M.D. Usibelli*, R. Lawrence-Berrey*, A. Pedersen* (1992). Adaptations in mesopelagic fishes. II. Protein contents of various muscles and actomyosin contents and structure of swimming muscle. Comp. Biochem. Physiol. 103B: 691-697
  • Gillett*, M.B., J.R. Suko*, F.O. Santoso*, P.H. Yancey (1997). Elevated levels of trimethylamine oxide in muscles of deep-sea gadiform teleosts: a high-pressure adaptation? J. Exper. Zool. 279:386-391 (see picture at right of gadiform fish)
  • Kelly*, R.H., P.H. Yancey (1999). High contents of trimethylamine oxide correlating with depth in deep-sea teleost fishes, skates, and decapod crustaceans. Biol. Bull. 196:18-25 ; PDF version here.
  • Yancey, P.H., J.F. Siebenaller (1999). Trimethylamine oxide stabilizes teleost and mammalian lactate dehydrogenases against inactivation by hydrostatic pressure and trypsinolysis. J. Exper. Biol. 202:3597-3603; news story: Dec. 11 '99 New Scientist (p.22)
  • Yin, M., H.R. Palmer, A.L. Fyfe-Johnson*, J.J. Bedford, R.A. Smith, P.H. Yancey (2000). Hypotaurine, N-methyltaurine, taurine, and glycine betaine as dominant osmolytes of vestimentiferan tubeworms from hydrothermal vents and cold seeps. Physiol. Biochem. Zool. 73:629.
  • Yancey, P.H., A.L. Fyfe-Johnson*, R.H. Kelly*, V.P. Walker*, M.T. Aunon* (2001). Trimethylamine oxide counteracts effects of hydrostatic pressure on proteins of deep-sea teleosts. J. Exp. Zool. 289:172
  • Yancey, P.H., W. R. Blake*, J. Conley* (2002). Unusual organic osmolytes in deep-sea animals: adaptations to hydrostatic pressure and other perturbants. Comp. Biochem. Physiol. A, 133 (3): 667-676 (click on vol. 133)
  • Fiess*, J., H.A. Hudson*, J.R. Hom*, C. Kato, P.H. Yancey (2002). Phosphodiester amine, taurine and derivatives, and other osmolytes in vesicomyid bivalves from cold seeps: correlations with depth and symbiont metabolism. Cahiers de Biologie Marine 43: 337-340
  • Yancey, P.H., M.D. Rhea*, D. Bailey, K. Kemp (2004). Trimethylamine oxide, betaine and other osmolytes in deep-sea animals: depth trends and effects on enzymes under hydrostatic pressure. Cell Molec. Biol. 50: 371-376
  • Rosenberg*, N.K., R.W. Lee, P.H. Yancey (2006). High contents of hypotaurine and thiotaurine in hydrothermal-vent gastropods without thiotrophic endosymbionts. J. Exp. Zool. 305A: 655-662.
  • Samerotte*, A.L., J.C. Drazen, G.L. Brand*, B.A. Seibel, P.H. Yancey (2007). Contents of trimethylamine oxide correlate with depth within as well as among species of teleost fish: an analysis of causation. Phys. Zool. Biochem. 80: 197-208
  • Brand*, G.L., R.V. Horak*, N. LeBris, S.K. Goffredi, S.L. Carney, B. Govenar, P.H. Yancey (2007). Hypotaurine and thiotaurine as indicators of sulfide exposure in bivalves and vestimentiferans from hydrothermal vents and cold seeps. Mar. Ecol. 28: 208-218.
  • Yancey, P.H., J. Ishikawa*, B. Meyer*, P. Girguis, R. Lee (2009). Hypotaurine and thiotaurine in polychaetes without endosymbionts from hydrothermal vents: correlation with sulfide exposure. J. Exp. Zool. 311A:439-447.
  • Herr, J.E., T.M. Winegard, M.J. O'Donnell, P.H. Yancey, and D.S. Fudge (2010). Stabilization and swelling of hagfish slime mucin vesicles. J. Exp. Biol 213: 1092-1099; news story here
  • Laxson*, C., N. E. Condon, J. C. Drazen, and P.H. Yancey (2011). Decreasing urea:methylamine ratios with depth in Chondrichthyes: A physiological depth limit? Physio. Biochem. Zool.84:494-505 online pre-publication
  • Jamieson, A., P.H.Yancey (2012). On the validity of the Trieste flatfish: dispelling the myth. Biol. Bull 222:171-175
  • Yancey, P.H., M. Gerringer*, A.A. Rowden, J.C. Drazen, A. Jamieson (2014). Marine fish may be biochemically constrained from inhabiting the deepest ocean depths. Proc. Natl. Acad. Sci. USA Mar. 3; see news story here


Giant rattail or grenadier (gadiform), 
Oregon slope, 2000m
ventmedium
A hydrothermal vent with tubeworms

C. OTHER: RNA in DEVELOPMENT; TOXICOLOGY; TEMPERATURE adaptations; MUSCLE physiology (*undergraduate co-authors)
  • Hough, B.R., P.H. Yancey*, E.H. Davidson (1973). Persistence of maternal RNA in Engystomops embryos. J. Exp. Zool. 185: 357-368
  • Somero, G.N., T.J. Chow, P.H. Yancey, C.B. Snyder (1977). Lead accumulation rates in tissues of the estuarine teleost Gillichthys mirabilis: salinity and temperature effects. Arch. Envir. Contam. Toxicol. 6: 337-346
  • Somero, G.N., P.H. Yancey, T.J. Chow, C.B. Snyder (1977). Lead effects on tissue and whole organism respiration of the estuarine teleost Gillichthys mirabilis. Arch. Envir. Contam. Toxicol. 6: 346-354
  • Yancey, P.H., G.N. Somero (1978). Temperature dependence of intracellular pH: its role in the conservation of pyruvate apparent Km values of vertebrate lactate dehydrogenases. J. Comp. Physiol. 125: 129-134
  • Altringham, J.D., I.A. Johnston, P.H. Yancey (1980). A sensitive positional feedback transducer for investigating the force-velocity relationship of actomyosin threads. J. Physiol. 9/12: 17P-18P
  • Yancey, P.H., I.A. Johnston (1982). Effect of electrical stimulation and exercise on the phosphorylation state of myosin light chains from fish skeletal muscle. Pflugers Archiv. 393: 334-339
  • Altringham, J.D., P.H. Yancey, I.A. Johnston (1980). Limitations in the use of actomyosin threads as model contractile systems. Nature 287: 338-340
  • Yancey, P.H., J.F. Siebenaller (1987). Coenzyme binding ability of homologs of M4-lactate dehydrogenase in temperature adaptation. Biochim. Biophys. Acta 924: 483-491

fish1

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III. MAMMALIAN/MEDICAL RESEARCH: KIDNEY and BRAIN OSMOLYTES (undergraduate co-authors*):
  • Yancey, P.H. (1988). Osmotic effectors in kidneys of xeric and mesic rodents: cortico-medullary distributions and changes with water availability. J. Comp. Physiol. 158B: 369-380
  • Wolff, S., P.H. Yancey, T.S. Stanton, R. Balaban (1989). A simple HPLC method for quantitating the major organic solutes of the renal medulla. Amer. J. Physiol. 256: F954-956
  • Yancey, P.H., M.B. Burg (1989). Distributions of major organic osmolytes in rabbit kidneys in diuresis and antidiuresis. Amer. J. Physiol. 257: F602-607
  • Yancey, P.H., M.B. Burg, S.M. Bagnasco (1990). Effects of NaCl, glucose and aldose reductase inhibitors on cloning efficiency of renal cells. Amer. J. Physiol. 258: C156-163
  • Yancey, P.H., M.B. Burg (1990). Counteracting effects of urea and betaine on colony-forming efficiency of mammalian cells in culture. Amer. J. Physiol. 258: R198-204
  • Yancey, P.H., R.G. Haner*, T. Freudenberger* (1990). Effects of an aldose reductase inhibitor on osmotic effectors in rat renal medulla. Amer. J. Physiol. 259: F733-F738
  • Edmands*, S., P.H. Yancey (1992). Effects on rat renal osmolytes of extended treatment with an aldose reductase inhibitor. Comp. Biochem. Physiol. 103C: 499-502
  • Peterson*, D.P., K M. Murphy*, R. Ursino*, K. Streeter*, P.H. Yancey (1992). Effects of dietary protein and salt on rat renal osmolytes: co-variation in urea and GPC contents. Amer. J. Physiol. 263: F594-F600.
  • Edmands*, S.D., K.S. Hughs*, S. Lee*, S.D. Meyer*, E. Saari, P.H. Yancey (1995). Time-dependent aspects of osmolyte changes in rat kidney, urine, blood and lens with sorbinil and galactose feeding. Kidney Int. 48: 344-353
  • Trachtman, H., P.H. Yancey, S.R. Gullans (1995). Cerebral cell volume regulation during hypernatremia in developing rats. Brain Res. 693: 155-62
  • Rohr*, J.M., S. Truong*, T. Hong*, P.H. Yancey (1999). Effects of ascorbic acid, aminoguanidine, sorbinil and zopolrestat on sorbitol and betaine contents in cultured rat renal cells. Exp. Biol. Online 4:3
  • Miller*, T., R. Hanson*, P.H. Yancey (2000). Developmental changes in organic osmolytes in prenatal and postnatal rat tissues. Comp. Biochem. Physiol. 125A:45-56 (click on vol. 125).
  • Bedford,, J.J., J. Schofield, P.H. Yancey, J.P. Leader (2002). The effects of hypoosmotic infusion on the composition of renal tissue of the Australian brush-tailed possum Trichosurus vulpecula. Comp. Biochem. Physiol. 132B: 645-652 (click on vol. 132).
  • Howard, M., H. Fischer, J. Roux, B. C. Santos, S.R. Gullans, P. H. Yancey, W. J. Welch (2003). Mammalian osmolytes and S-nitrosoglutathione promote delta-F508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein maturation and function. J. Biol. Chem. 278: 35159 - 35167
  • Stein, C.S., P.H. Yancey, I. Martins, R.D. Sigmund, J.B. Stokes, B.L. Davidson (2010). Osmoregulation of CLN3 in the renal medulla. Am. J. Physiol. Cell Physiol. 298: C1388-C1400

Normal kidney cells growing
in tissue culture
ibuprofen cells
Kidney cells in culture exposed to 1mM Ibuprofen
cystic fibrosis treatment
Restoring cystic-fibrosis channel function
with osmolytes (Howard et al., 2003)

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